I have also used commercial packages such as ImagePro plus and Leica QWin. If no one on your research team has experience in IA, try to find somebody who does to get you going. ![]() However, i have almost 30 years of experience in image analysis (IA) and am a reasonable programmer as well as a biologist. I find Fiji very good and it does do most of what I want, and has a very helpful user community. Also, as it's fully open source, it allows me to give away software that i develop using ImageJ/Fiji. ![]() I mostly use Fiji, a version of ImageJ, as it is free and contains a lot of plugins as standard that I find useful.
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